長(cháng)春光機所：1500米級深海生物(wù)基因測序儀海試成功

發布時間：2024-07-12

日前，記者從中(zhōng)國(guó)科(kē)學(xué)院長(cháng)春光機所獲悉，由該所吳一輝研究員團隊聯合中(zhōng)國(guó)科(kē)學(xué)院深海科(kē)學(xué)與工(gōng)程研究所杜夢然研究員團隊，研制的1500米級深海生物(wù)基因測序儀海試成功。

深海生物(wù)基因測序儀參加了“探索二号”TS2-38-1航次南海科(kē)考作(zuò)業，測序儀搭載深海原位實驗室完成兩次布放，最大潛深1380米。首次連續測序8小(xiǎo)時，第二次完成文(wén)庫構建、固相擴增到基因測序全流程，連續工(gōng)作(zuò)30小(xiǎo)時。兩次測序數據質(zhì)量Q30分(fēn)别為(wèi)92%和95%，成功識别出中(zhōng)國(guó)計量科(kē)學(xué)研究院提供的97條DNA樣本。

五年來，科(kē)研團隊突破了測序方法、基因大數據處理(lǐ)、文(wén)庫芯片、多(duō)色光學(xué)聚焦成像、精(jīng)準流動定量、多(duō)重動态溫控、水下化封裝(zhuāng)等關鍵部件及自糾錯軟件等難題，初步實現了從文(wén)庫構建到測序的全流程無人值守自動化。

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